P-99: Survey of Noscapine Effect on Apoptosis of Human Endometrial Stromal Cells: An In Vitro Study

Background: Noscapine is a water soluble alkaloid with antitussive property and very less toxicity. It is obtained from the Papaveraceae family. Recently, new pharmacological properties such as anticancer, anti stroke and anti-angiogenic effects are introduced. There is no scientific report on noscapine effect on reproductive system. The aim of present study was to determine in vitro effect of noscapine on apoptosis of endometrial stromal cells in in vitro culture. Materials and Methods: In this experimental study, normal endometrial biopsy (n=8) were collected in sterile condition and chopped by sterile blade. Stromal cells were isolated after collagenase digestion (2mg/ml); cell filtration through filter mesh and ficoll layering. The cells were divided into 5 groups; control, 10, 25, 50 and 100 μM noscapine concentrations, and were cultured for 48 hours in DMEM/F12 containing FBS (5%) and antibiotics. Cell apoptosis was determined by TUNEL Staining . Data was analyzed by one way-ANOVA test and p< 0.05 was significant. Results: Endometrial stromal cell apoptosis were 2.32, 3.19, 3.7, 6.15, 8.49%, in control, 10, 25, 50 and 100 μM of noscapine concentration respectively. The difference between groups was significant (p= 0.004). Conclusion: Stromal cell apoptosis were increased by noscapine in a dose dependent manner.